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E306 and S333 mutations affect cytoskeleton remodelling. Epifluorescence images of COS7 cells transfected with (A) EYFP (enhanced yellow fluorescent protein)-E306A, (B) EYFP-E306D, (C) EYFPs333A and (D) EYFP-S333D. Cells were co-stained with Alexa Fluor® 594 phalloidin. Overlay images show colocalization of EYFP-E306D and EYFP-S333D mutants with actin in cell protrusions. Scale bar, 15 μm.
