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. 2005 Jul 15;6(8):729–735. doi: 10.1038/sj.embor.7400465

Figure 1.

Figure 1

Effect of ICRF-193 on chromatin assembly during replication of sperm nuclei in egg extracts. Sperm nuclei were incubated in lowspeed supernatants at 1,000 nuclei/μl in the presence of 150 μM ICRF-193, or drug solvent alone (dimethylsulphoxide (DMSO)). Replicating DNA was pulse labelled for 1 min, then the label was chased for the indicated time and nucleosome assembly on newly replicated DNA was monitored by micrococcal nuclease digestion and gel electrophoresis. Consecutive lanes correspond to increasing digestion times (grey triangles). Density scans of representative lanes are shown in red (ICRF-193) and black (DMSO).