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. 2005 Jul 15;6(8):775–781. doi: 10.1038/sj.embor.7400466

Figure 3.

Figure 3

FCR3Δvar2csa mutants show no chondroitin sulphate A (CSA)-specific cytoadhesion after selection on CSA-expressing cell lines and recombinant human thrombomodulin. Mean±s.d. of IE adhering per square millimetre (IE/mm2) for four different fields is shown (A,B). (A) Selection of FCR3Δvar2csa mutants and parental FCR3 parasites on recombinant human thrombomodulin. Erythrocytes infected with FCR3-CSA, FCR3-CD36, 1F1 and 2A5 were selected four times on recombinant human thrombomodulin-CSA coated to Petri dishes. (B) Selection of FCR3Δvar2csa mutants and parental FCR3 parasites on Sc1707. Trophozoite-stage Plasmodium falciparum clones FCR3-CSA, FCR3-CD36, 1F1 and 2A5 were subjected to repeated rounds of selection over Sc1707 cells, followed by evaluation of adhesion to Sc1707. (C,D) Adhesion profiles of P. falciparum IE after selection on Sc1707 cells. Trophozoite-stage P. falciparum parasites FCR3-CSA, FCR3-CD36, 1F1 and 2A5 were subjected to repeated rounds of selection over Sc1707 cells, followed by evaluation of adhesion to CSA-coated (C) and CD36-coated (D) plastic Petri dishes. Adhesion after selection is shown for FCR3-CSA1707, FCR3-CD361707, 1F11707 and 2A51707. Data are the mean±s.e.m. of IE per square millimetre adhering to CSA- and CD36-coated plastic Petri dishes, as determined in three independent assays.