. 2005 Jul 22;6(8):736–741. doi: 10.1038/sj.embor.7400467

Table 1.

Growth phenotypes, DnaA content and marker frequencies of strains cultured in LB medium

Strain	Relevant genotype	τ^{^a} (min)	OD_{600 nm} in stationary phase	Marker frequencies^{^b}		DnaA/total protein^{^c}
				*oriC/ter*	*dnaA/xasA*
MG1655	Wild type	26	7.74	1.9	2.0	1.00±0.25
JC125	Δhda	30	5.70	4.1	3.9	1.15±0.22
JC126	ΔdatA	26	6.18	2.0	1.9	1.08±0.08
JC326	ΔseqA	36	4.02	2.1	2.3	1.79±0.33

^aLog-phase growth doubling time.

^bAbundance of oriC relative to ter, and dnaA relative to xasA, as determined by Southern blot analysis.

^cBased on immunoblots in Fig 3. Only experimental values (n=3–5) that fell within the range of the corresponding DnaA standard curves were used for further calculations.

DnaA content was calculated as DnaA/total protein and all values were normalized to the value for wild-type cells (MG1655), which was given an arbitrary value of 1.00.