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. 2005 Aug 19;6(10):942–948. doi: 10.1038/sj.embor.7400491

Figure 2.

Figure 2

Internalization of epidermal growth factor receptor after treatment with PD158780. (A) BT20 cells were treated with PD158780 (100 nM) for 15 min and then stimulated with epidermal growth factor (EGF) (100 ng/ml) at 37°C for the indicated time. EGF receptor (EGFR; green) and phospho-tyrosine (pTyr; red) or phospho-EGFR (pEGFR; red) localization was determined by double indirect immunofluorescence. (B) BT20 cells were treated with PD158780 (100 nM) for 15 min and then stimulated with EGF (100 ng/ml) at 37°C for the indicated time. EGFR (green) and EEA1 (red) localization was determined by double indirect immunofluorescence. (C) Quantitative analysis of the effects of PD158780 on EGFR internalization in BT20 cells by flow cytometry. Data are the means of at least three experiments performed in triplicate. Scale bars, 20 μm.