Figure 3.

Epidermal growth factor-induced receptor dimerization in the inhibition of EGFR kinase. (A) EGF-induced dimerization of K721A–YFP (yellow fluorescent protein). 293T cells were transiently transfected with EGFR–YFP or K721A–YFP. After EGF stimulation for 15 min, 293T cells were crosslinked with bis-(sulphosuccinimidyl suberate) (BS³). The cell lysates were immunoblotted with an anti-EGFR antibody. (B) EGF-induced EGFR dimerization in BT20 cells treated with either PD158780 or AG1478. BT20 cells were treated with either PD158780 or AG1478, and then stimulated with EGF for 15 min. The cells were crosslinked with bis-(sulphosuccinimidyl suberate) (BS³) and the cell lysates were immunoblotted with an anti-EGFR antibody.