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. 2005 Sep 2;6(10):961–967. doi: 10.1038/sj.embor.7400509

Figure 2.

Figure 2

TRBP and Dicer co-sedimentation on glycerol gradients. (A,B) Sedimentation of cytoplasmic extracts from P19 cells (A) and human embryonic kidney (HEK)293 cells (B). Gradient fractions were analysed for Dicer and TRBP (human immunodeficiency virus (HIV-1) transactivating response (TAR) RNA-binding protein) by western blotting and for miR-17 by northern blotting. (C) Activity of fractions analysed in (B), pooled as indicated (top of the panel), in processing 30-bp double-stranded RNA into small interfering RNA.