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. 2005 Aug 26;6(10):968–972. doi: 10.1038/sj.embor.7400510

Figure 1.

Figure 1

Calicivirus VPg interacts with eIF4E. (A) His-tagged feline calicivirus (FCV) VPg coupled to NTA-agarose beads was incubated with HeLa S10 extracts. Complexes were isolated by lowspeed centrifugation and proteins bound were analysed for the components of the eIF4F complex by western blot. (B) VPg interaction with murine eIF4E and eIF4G, as shown by enzyme-linked immunosorbent assay-based binding assay. Wells were precoated with 1.0 μg of untagged VPg from both FCV and Lordsdale virus (LDV) and incubated with increasing amounts of an Escherichia coli lysate expressing comparable levels of glutathione S-transferase (GST)–eIF4E or GST–eIF4G. Complexes were detected using anti-GST antibodies. OD, optical density.