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VPg interacts with eIF4E in infected cells. Feline kidney cells were mock infected or infected with feline calicivirus and extracts were prepared 5 h after infection. Extracts were incubated with either cap–Sepharose (A), as described (Ptushkina et al, 1999), or Sepharose 4B (B) and bound proteins were analysed by Western blot with antisera to VPg, p39 or eIF4E.
