Figure 1.

Identification of new immune deficiency pathway components. (A) Effect of depletion of candidate transcript on mtk reporter induction. Depletion of known and new immune deficiency (IMD) pathway components by RNA interference (RNAi) leads to a significant reduction of IMD–Rel pathway activity (black bars). Zscores were calculated as the number of median-adjusted standard deviations that a particular RNAi experiment differed from the median of all RNAi experiments in a 96-well plate. (B) Depletion of known and newly identified components leads to a reduction of the IMD pathway-dependent attacineA (attA) reporter. The percentage reduction of pathway activity after Escherichia coli stimulation as compared with a control double-stranded RNA (dsRNA; green fluorescent protein (GFP)) is shown. The pathway-specific reporter is normalized against a constitutive co-reporter. Error bars represent the standard deviation of three experiments.