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. 2005 Dec 2;7(2):212–218. doi: 10.1038/sj.embor.7400593

Figure 3.

Figure 3

Mre11 localization at HO breaks in the absence or excess of Sae2. YEP+raf nocodazole-arrested cell cultures of wild-type (wt) JKM139 and isogenic sae2Δ and GAL-SAE2 strains, all carrying an MRE11-MYC-tagged allele at the MRE11 locus, were transferred to YEP+raf+gal in the presence of nocodazole (time zero). Cell samples taken at the indicated times were processed for staining with 4,6-diamidino-2-phenylindole (DAPI) and anti-Myc antibody indirect immunofluorescence. (A) Representative fields were photographed at the indicated times. (B) Kinetics of Mre11 foci formation were determined by scoring 200 cells for each strain at each time points. (C) Wild-type JKM139 and isogenic sae2Δ, rad50s, mre11-H125N, mre11-D56N, mre11Δ and mre11Δ sae2Δ strains, exponentially growing in YEP+raf, were transferred to YEP+raf+gal. Protein extracts from samples taken at the indicated times were analysed by western blot with anti-Rad53 antibodies.