Figure 5.

Complex transcriptional control of wc-1. (A) Expression of wc-1 by P_dist and P_prox is required for entrainment. Race tubes were inoculated with the indicated strains and exposed to 12 h:12 h light:dark cycles. Conidial densities were plotted versus time. Phases of conidiation peaks were calculated as zeitgeber time (h; mean±s.e.m.). (B) Regulation of P_dist and P_prox by the WCC. Upper panel: schematic outline of the wc-1 gene. P_dist and P_prox are indicated. A putative CAAT box, TATA box and initiator sequences are shown. The grey boxes (Box 1–3) indicate putative GATN-type binding sites for WCC. Lower panel: sequence comparison of wc-1 (Box 1–3) and the light-responsive elements of frq (Froehlich et al, 2002) and al-3 (Carattoli et al, 1994). The arrows indicate the orientation of GATN elements.