Fig. 5. Primer extension and RLM-RACE analysis of BEV sg RNAs 2–5. RLM-RACE products obtained for mRNAs 2–5 (see Figure 3) were subjected to sequence analysis with oligonucleotide primers 1403, 1404, 1405 and 1406, respectively. In addition, primer extension analysis was performed with the respective oligonucleotides on mRNAs 2–4. Sequence reaction mixtures and primer extension products were run alongside in 6% urea–polyacrylamide gels. Arrows indicate the 5′-most residues of each mRNA as predicted by primer extension and/or RLM-RACE. The nucleotide sequence is presented, with adaptor- derived residues shaded in gray.