Fig. 5. MIP-2 mRNA is bound by hnRNP A0 in extracts from LPS-stimulated cells. (A) RAW cells were left untreated or treated for 1 h with 50 ng/ml LPS and lysed. Then immunoprecipitation with (lanes 3–4) or without (lanes 1–2) the hnRNP A0 antibody was carried out from clarified extracts and the RNA bound identified using a differential display-based assay with random primer set 1 (see Materials and methods). CTL, control experiments. (B) Sequences of part of the 3′-UTR of murine MIP-2 mRNA (accession No. X53798) and TNF-α mRNA (accession No. X02611).