TABLE 2.
Viral entry into CCR5+ and CXCR4+ target cells mediated by mutant envelope glycoproteinsa
| Protein | Entry into:
|
|||
|---|---|---|---|---|
| CCR5+ cells
|
CXCR4+ cells
|
|||
| −PA14 | +PA14 | −12G5 | +12G5 | |
| JR-FL | +++ | − | − | |
| JJL | − | ++ | − | |
| JJΔ | − | − | ||
| JLL | − | ++ | − | |
| JLJ | + | − | − | |
| JLΔ | − | − | ||
| LAI | − | +++ | − | |
| LLJ | ++ | − | − | |
| LLΔ | − | − | ||
| LJJ | +++ | − | − | |
| LJL | − | +++ | − | |
| LJΔ | − | − | ||
a
U87 CD4+ cells expressing CCR5 or CXCR4 were infected with NL luc+ env− virions (100 to 200 ng of p24/ml) pseudotyped with mutant envelope glycoproteins and expressing the luciferase gene. Infections of CCR5+ cells were carried out in the presence or absence of MAb PA14 (10 μg/ml), whereas infections of CXCR4+ cells were carried out in the presence or absence of MAb 12G5 (10 μg/ml). −, rlu >150; +, rlu between 5 × 103 and 1 × 104; ++, rlu between 104 and 105; +++, rlu > 105.