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. 2002 Sep;76(17):8966–8972. doi: 10.1128/JVI.76.17.8966-8972.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 5. — Sequence and growth characteristics of variant RPδ6/SIV_p17(21). (A) The amplified PCR product obtained through RT-PCR from total cellular RNA prepared from the fifth consecutive passage of RPδ6/SIV_p17 in HeLa cells (Fig. 4B) was subjected to sequence analysis. The variant virus had acquired a duplication of IRES and insert sequences (duplicated sequences are boxed in gray). The duplication was in frame, containing two tandem AUG codons in Kozak context (capital letters). (B) RPδ6/SIV_p17(21) virus (filled diamonds) exhibited propagation kinetics in HeLa cells that resembled those of PVS-RIPO (open squares). p.i., postinfection.

FIG. 5. — Sequence and growth characteristics of variant RPδ6/SIV_p17(21). (A) The amplified PCR product obtained through RT-PCR from total cellular RNA prepared from the fifth consecutive passage of RPδ6/SIV_p17 in HeLa cells (Fig. 4B) was subjected to sequence analysis. The variant virus had acquired a duplication of IRES and insert sequences (duplicated sequences are boxed in gray). The duplication was in frame, containing two tandem AUG codons in Kozak context (capital letters). (B) RPδ6/SIV_p17(21) virus (filled diamonds) exhibited propagation kinetics in HeLa cells that resembled those of PVS-RIPO (open squares). p.i., postinfection.