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Gel filtration analysis of SOSIP gp140. (A) SOS gp140 and SOSIP gp140 (SOS I559P) were fractionated on a Superdex 200 column. The individual fractions were analyzed by SDS-PAGE and Western blotting. (B) The SOSIP gp140 fractions from panel A were analyzed by BN-PAGE and Western blotting. The elution positions (peak fractions) of standard proteins are indicated.
