FIG. 2.

Specificity of LM-PCR for molecules with blunt ends. (A) The specificity of LM-PCR for blunt targets representing completed reverse transcripts (top) or asymmetric targets representing the processed form of the HIV-1 genome (bottom) was evaluated with both blunt linkers and linkers with complementary overhangs. (B) Ten thousand copies of a linear molecule with blunt (StuI restricted) or asymmetric ends (AccI restricted) were loaded into a LM-PCR mixture. Ligation of the linker onto the terminus of the plasmid was evaluated with a 28-cycle PCR employing oligonucleotide 25t and LPCR-L. Numbers refer to the reactions shown in panel A.