TABLE 1.
Half-life measurements for preintegration latency in infected resting CD4+ T cellsa
| Expt no. | MOIb | Acid treatmentc | Decay rated (days−1) | Half-lifee (days) | R2 of regression |
|---|---|---|---|---|---|
| 1 | 2 | No | 0.59 | 1.2 | 0.99 |
| 2 | 5 | No | 0.78 | 0.89 | 0.86 |
| 3 | 7 | No | 1.0 | 0.69 | 0.98 |
| 4 | 10 | Yes | 0.82 | 0.84 | 0.88 |
| 5 | 10 | Yes | 0.78 | 0.89 | 0.98 |
| 6 | 20 | Yes | 0.96 | 0.72 | 0.89 |
a
Highly purified resting CD4+ T cells were infected with NL4-1-GFP and incubated for various times up to 3 days before activation and analysis of induced GFP expression.
b
MOI, multiplicity of infection.
c
In some experiments, infected cells were washed briefly at an acidic pH to promote direct fusion of the virus with the plasma membrane.
d
Decay rates were determined by modeling the decay data from the first 3 days postinfection as an exponential process.
e
The average half-life for the six experiments was 0.9 ± 0.2 days.