FIG. 1.
Construction of PrV UL46 and UL47 deletion mutants. (A) A schematic map of the PrV genome shows the long (UL) and short (US) unique regions, the inverted repeat sequences (IR, TR), and the positions of BamHI restriction sites. Numbers indicate BamHI restriction fragments. (B) Coding regions of proteins in the genomic region relevant for this study. Arrows indicate transcriptional orientations. Also indicated is a region of repeated sequences between the UL27 and UL46 genes, as well as location of consensus sequences for polyadenylation (polyA) and relevant restriction sites. (C) Location of primers used for PCR amplification (see Table 1). (D) Genotypes of the constructed viral mutants.