FIG. 6.

Neuraminidase treatment of genital (Chang C, HeLa, SiHa) and corneal (HCE) cells protects from infection by Ad37 (A) but not by Ad5 (B). Adherent cells were preincubated with or without neuraminidase and then with virion dilutions corresponding to 5,000 FFU/sample, permitting attachment to cells but not internalization. Thus, the virion dilutions were adjusted in order to obtain 100 FFU/viewing field in the control wells (nontreated cells). Then, unbound virions were removed by washing and incubated at 37°C, thus permitting internalization of virions in cells. At 40 h postinfection, the cells were rinsed, fixed, stained, and examined in an immunofluorescence microscope (C) as described in Materials and Methods. The results are presented as the means from three independent experiments. CTRL, control.