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Release of virions by Arg183 mutant viruses and by parental virus. CEF cultures were infected at an MOI of 100 at 30°C and maintained at 30°C (A) or shifted to 40°C at 1 h p.i. (B). At 2-h intervals, the medium was removed and the cells were refed with the same volume of fresh medium. The viral titer was determined by plaque assays performed at 30°C on CEF cells. ▴, parental Toto virus; ▪, Lys183 virus; ♦, Ala183 virus; •, Ser183 virus.
