FIG. 6.

Ad-mediated gene transfer to CHO, CHO/VPAC1, and CHO/VPAC2 cells. CHO, CHO/VPAC1, and CHO/VPAC2 cells were preincubated for 10 min with either DMEM-F-12 medium or the same medium containing recombinant Ad5 knob protein at a concentration of 100 μg/ml prior to infection for 30 min with Ad5Luc.NNVIP viruses (MOI of 40 viral particles per cell). Luciferase activity was determined in cell lysates 20 h postinfection. Each data point corresponds to an average of three measurements. Numbers at the bottom indicate the length of the penton base-derived sequences flanking the site of VIP incorporation. “wt” corresponds to the Ad5Luc1 control virus bearing unmodified Ad5 fibers.