FIG. 8.
Analysis of binding of Ad5Luc.10VIP and Ad5Luc.10VIP18-23 viruses to CHO/VPAC1 cells. [3H]thymidine-labeled viruses were incubated for 1 h with cells at an MOI of 3,500 viral particles per cell. Unbound virions were then removed by washing the cells with the virus-free medium, and the number of particles bound per cell was calculated based on the specific radioactivities of the virions. Each data point corresponds to an average of three measurements. Cell attachment of the labeled Ad5Luc1 vector, which was used as a control, is shown by the “no ligand” bar.