TABLE 2.
Induction of monokine-specific transcripts in TAP-1 and HL-60 cells by MV strains and recombinants
| Monokine | Monokine mRNA level after stimulation witha:
|
||||||
|---|---|---|---|---|---|---|---|
| Mock | ED | ED(WTF-F) | ED(WTF-H) | ED(WTF-H;N→Y) | ED(WTF-F/H) | WTF | |
| IL-12 p40 | 0.5 ± 1.3 | 1.8 ± 0.9 | 1.7 ± 1.1 | 12.1 ± 5.5 | 2.0 ± 2.2 | 9.9 ± 1.3* | 10.6 ± 2.7* |
| IL-1α | 0.9 ± 4.2 | −2.2 ± 3.7 | −1.1 ± 3.5 | 12.2 ± 2.2 | 0.2 ± 4.2 | 25.6 ± 3.9* | 29.6 ± 6.2* |
| IL-1β | 1.5 ± 0.6 | 0.0 ± 2.1 | −0.8 ± 2.4 | 36.6 ± 2.8* | 1.0 ± 2.9 | 40.2 ± 1.2* | 58.8 ± 1.5* |
| IL-1 Ra | 7.1 ± 3.3 | 2.6 ± 1.3 | 12.4 ± 1.7 | 66.4 ± 14.8 | 9.9 ± 1.2 | 67.4 ± 2.3* | 55.3 ± 5.4* |
| IL-6 | 1.7 ± 1.7 | 0.3 ± 0.7 | 0.4 ± 1.4 | 21.1 ± 1.3* | 1.7 ± 1.0 | 20.7 ± 2.7* | 30.1 ± 2.7* |
a
Levels of monokine-specific mRNAs were determined in vitamin D3-treated THP-1 cells 12 h after stimulation by RPA and normalized to those of the L32 housekeeping gene. Values were determined relative to induction by PamCSK (set as 100%) as means of three independent experiments (standard deviations are indicated [P < 0.01]). Statistically significant differences from the control are indicated by asterisks.