Digitally scanned electron micrograph of thin sections of wild-type (WT) HSV-1(F)-infected Vero cells harvested and fixed 14 to 18 hpi. The nucleus, INM, ONM, and cytoplasm are labeled in panels A and B. Gold beads associated with the UL31 protein (pUL31) are demarcated with arrowheads. Panel C demonstrates extracellular virions devoid of pUL31-specific immunogold label. For reference, in the electron micrographs presented here, HSV-1 nucleocapsids are approximately 120 nm in diameter and gold beads are 12 nm in diameter. Original magnification of panels A to C, ×45,000.