FIG. 4.
Digital confocal images showing localization of the UL34 and US3 proteins in HEp-2 cells infected with HSV-1(F) or US3-null HSV-1. Subconfluent monolayers of HEp-2 cells were infected for 12 h with either US3-null mutant virus R7039 (A to C) or HSV-1(F) (D to F). Formaldehyde-fixed cells were immunostained with chicken anti-UL34 antibody that was detected with donkey anti-chicken IgG-Texas Red conjugate (red), and rabbit anti-US3 antibody was detected with goat anti-rabbit IgG-fluorescein isothiocyanate conjugate (green). The long white arrow indicates US3 protein detected at the plasma membrane, and the white arrowhead indicates US3 protein detected at the nuclear membrane. Areas of colocalization of the two proteins appear yellow in the merged images (C and F). Original magnification, ×1,000.