TABLE 1.

Summary of the distribution of U_L31 protein determined by immunogold analysis of thin sections^a

Virusb	Total no. of gold beads counted in 10 whole-cell sections	No. (%) of gold beads in:
		Nuclear membrane	Nucleoplasm	Cytoplasm
Wild type	312	21.6 ± 3.0 (69.2)	5.4 ± 0.7 (17.3)	4.2 ± 1.0 (13.5)
UL34 null	1,104	38.1 ± 4.5 (34.5)	37.0 ± 5.2 (33.5)	35.3 ± 6.7 (32.0)
US3 null	2,295	127.7 ± 29.6 (55.6)	52.7 ± 10.0 (23.0)	49.1 ± 14.8 (21.4)
UL31 null	39	0.6 ± 0.2 (15.4)	2.4 ± 0.7 (61.5)	0.9 ± 0.3 (23.1)

^aThe total numbers of gold beads in 10 randomly selected whole-cell sections of wild-type virus [HSV-1(F)], U_L31-null virus-, U_L34-null virus-, and U_S3-null virus-infected cells were quantified. The mean number of U_L31 protein-specific gold beads and the standard error of each mean are indicated for three regions of the cells. The following formula was used to calculate the percentage of gold beads in a given region of the cell infected with the indicated virus: (number of gold bead particles per region of 10 whole-cell sections/total number of gold bead particles in 10 whole-cell sections) × 100 = % of gold beads per region.

^bTen samples of each virus were tested