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trans complementation of P25K:FLAG processing in wild-type VV-infected cells. Cells were infected with wild-type VV and transfected with pRB21:17L, p25K:FLAG, or a combination of these, and cleavage of the P25K:FLAG substrate was determined by Western blotting using anti-FLAG MAb. Lane 1, cells alone; lane 2, wild-type VV; lane 3, VV with I7L; lane 4, VV with P25K:FLAG; lane 5, VV with I7L and P25K:FLAG.
