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. 2002 Sep;76(17):8667–8674. doi: 10.1128/JVI.76.17.8667-8674.2002

TABLE 2.

Amounts of mutant and wild-type proteinase and cleavage products in nuclei and NIEPsa

Protein NP-40 nuclei
NIEPs
PSL for:
I/Wt PSL for:
I/Wt
Wtb I Wtc I
Precursors (pNP1 + NP1) 105 183 1.7
Assemblin 135 303 2.2 424 1,050 2.5
Cn 145 719 4.9 286 1,614 5.6
An 202 2,576
Totald 482 1,022 2.1 3,286 2,664 0.8
a

Calculations were made from quantification of data shown in Fig. 7, lanes 4 to 6 (for NP-40 nuclei [pellet fraction of NP-40-treated cells]), and Fig. 8, lanes 1 and 4 (for NIEPs). Values are in units of photostimulated luminescence (PSL).

b

Values for the wild type (Wt) were increased 1.04 fold (1,547/1,484 PSL units) to normalize (relative to the amount of mCP) between the samples for NP-40 nuclei from Wt and mutant (I) virus-infected cells (immunoassay data not shown).

c

Values for the Wt were increased 1.37 fold (7,665/5,610 PSL units) to normalize (relative to the amount of mCP) between the samples from Wt and I NIEPs.

d

Total is sum of values for assemblin plus Cn plus An.