TABLE 2.
Amounts of mutant and wild-type proteinase and cleavage products in nuclei and NIEPsa
| Protein | NP-40 nuclei
|
NIEPs
|
||||
|---|---|---|---|---|---|---|
| PSL for:
|
I−/Wt | PSL for:
|
I−/Wt | |||
| Wtb | I− | Wtc | I− | |||
| Precursors (pNP1 + NP1) | 105 | 183 | 1.7 | |||
| Assemblin | 135 | 303 | 2.2 | 424 | 1,050 | 2.5 |
| Cn | 145 | 719 | 4.9 | 286 | 1,614 | 5.6 |
| An | 202 | 2,576 | ||||
| Totald | 482 | 1,022 | 2.1 | 3,286 | 2,664 | 0.8 |
Calculations were made from quantification of data shown in Fig. 7, lanes 4 to 6 (for NP-40 nuclei [pellet fraction of NP-40-treated cells]), and Fig. 8, lanes 1 and 4 (for NIEPs). Values are in units of photostimulated luminescence (PSL).
Values for the wild type (Wt) were increased 1.04 fold (1,547/1,484 PSL units) to normalize (relative to the amount of mCP) between the samples for NP-40 nuclei from Wt and mutant (I−) virus-infected cells (immunoassay data not shown).
Values for the Wt were increased 1.37 fold (7,665/5,610 PSL units) to normalize (relative to the amount of mCP) between the samples from Wt and I− NIEPs.
Total is sum of values for assemblin plus Cn plus An.