TABLE 1.

CXCR4 Primer Sets and Experimental Conditions for PCR Amplification and DGGE

			Temperature (°C)
Fragment	Forward Amplimer, 5′-3′	Reverse Amplimer, 5′-3′	Size (bp)	Melting	Annealing
A	CTCCAGTAGCCACCGCATCT	[40GC]GCTGCGCTCTAAGTTCAAACG	154	73	62
B	[40GC]GAATGTCCATTCCTTTGCCTCT	GCCTGTACTTGTCCGTCATGC	286	73	60
C	[40GC]CCACCATCTACTCCATCATC	AGACGCCAACATAGACCAC	397	66	55
D	CACGCCACCAACAGTCAGA	[40GC]AGCAGGACAGGATGACAATACC	278	71	60
E	[40GC]CAGTTTCAGCACATCATGGT	AGGATGAGGATGACTGTGGT	180	66	55
F	CATCTCCAAGCTGTCACACT	[40GC]TTACATCTGTGTTAGCTGGAGT	445	66	54
G	TCCACTGAGTCTGAGTCTTCAA	[40GC]TCCTGCCTAGACACACATCA	282	67	54

GC-clamp used was as follows: [40GC] CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCG.