FIG. 1.

Selective induction of BRLF1 and BZLF1 expression by inducing chemicals in B95-8 and HH514-16 cells. (A) Induction of mRNAs containing BRLF1 and BZLF1. At 20 h after treatment of the cells with TPA or n-butyrate, singly or in combination, RNA was prepared by the TRIzol method. RNA from 3 × 10⁶ cells per lane was analyzed by Northern blotting with a probe prepared from a portion of the BZLF1 cDNA. This probe detects the 4.0- and 3.0-kb bicistronic (BRLF1 plus BZLF1) mRNA from Rp and the 1.0-kb monocistronic (BZLF1) mRNA from Zp (37). The blot was reprobed with β-actin to control for loading of RNA. (B) Comparison of the effects of n-butyrate (B) and TSA. Cytoplasmic RNA prepared 17 h after chemical treatment was analyzed by Northern blotting with the BZLF1 probe. The RNA blots were also probed for β-actin and the H1 RNA component of human RNase P to control for RNA loading.