FIG. 2.

Generation and analysis of sibling cultures derived from pool 5. (A) Flow chart showing the protocol for generating pools 5, 5-H1, 5-H5, 5-B1, and 5-B5. L, H, C, and B in the circle represent LTL-lox transduction, HAT selection, Cre expression, and BVdU selection, respectively. (B) Relative frequencies of various integration targets obtained from different pools. Bars indicate the ratio of the number of sister plasmid clones representing each integration target to the total number of the obtained clones shown on the right.