FIG. 2.

HPLC analysis of sucrose density gradient fractions from an SIV CP-MAC purification. Cell culture supernatants were processed as described in Materials and Methods. Fractions were collected from the sucrose density gradient after centrifugation. The percent sucrose was determined for each fraction. A 25-ml sample from each fraction was diluted 1:3 with TNE buffer and centrifuged at 100,000 × g to pellet any virus that may have been present. The pellets were resuspended in 8 M guanidine-HCl and analyzed for protein composition by HPLC under nonreducing conditions.