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. 2002 Jun;76(11):5315–5325. doi: 10.1128/JVI.76.11.5315-5325.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — HPLC analysis of HIV-1 MN/H9 cl.4. After purification, virions were disrupted in 8 M guanidine-HCl and separated by HPLC. Peaks were detected at 280 nm. Proteins eluted from the column with an acetonitrile gradient were analyzed by SDS-PAGE, immunoblot, mass spectrometry, protein sequencing, and amino acid analysis. After gp120 and gp41 were identified on the HPLC profile by immunoblot, fractions containing SU and TM were analyzed by SDS-PAGE followed with silver staining (insertions on HPLC picture).