FIG. 10.
The Bcl-xL NF-κB element competes very poorly for RelB-p50 binding to the URE. Cultures (P-150 dish) of NIH 3T3 cells were transfected with 10 μg of vector expressing RelB plus 5 μg of vector expressing p50 protein. After 48 h, nuclear extracts were prepared, and samples (5 μg) were subjected to EMSA, using the URE NF-κB element as a probe, in the absence (none) or presence of 10 or 100× molar excess of the indicated unlabeled oligonucleotide as competitor DNA. The resulting autoradiogram was subjected to densitometry, and the RelB-p50 complexes present are given below as percents relative to the control.