FIG. 2.

IE1 induces E8-CAT c-myc URE NF-κB element-driven TK promoter activity in SMC and NIH 3T3 cells. Cultures of either SMCs (A) or NIH 3T3 cells (B) were transiently transfected, in triplicate, with the indicated amounts of pON2205 vector expressing IE1 protein, 0.5 μg of E8-CAT vector, 0.5 μg of SV40 β-Gal expression vector, and enough pBluescript to make a total of 2.5 μg of DNA. After 48 h, the cells were harvested, and CAT activity and β-Gal activity were measured. The data presented are the mean of four separate experiments. Baseline untreated E8-CAT activity was set at 1. The results are expressed as mean plus standard error of the mean.