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CMV IE1 protein induces nuclear levels of RelB and p50 proteins. Cultures (P-100 dish) of either SMCs or NIH 3T3 cells were transfected with 10 μg of pON2205 vector expressing IE1 protein (+) or pCMV empty vector DNA (−). After 48 h, cytoplasmic and nuclear lysates were prepared and samples (30 μg) were subjected to immunoblot analysis for IE1, RelB, p50 and p105, RelA, c-Rel,and β-actin, as indicated.
