FIG. 6.

CMV IE1 fails to induce NF-κB activity in relB^−/− MEFs. Cultures of relB^−/− MEFs at 50 to 70% confluence were transiently transfected, in triplicate, with 0, 0.25, 0.5, or 0.75 μg of either pON2205 vector expressing IE1 protein (A) or RelA expression vector plus 0.5 μg of p50 expression vector (B) and 0.5 μg of E8-CAT reporter vector, 0.5 μg of SV40 β-Gal expression vector, and enough pBluescript to make a total of 2.5 μg of DNA. After 48 h, the cells were harvested, and CAT activity and β-Gal activity were measured. The data presented are the mean of two separate experiments. Baseline untreated E8-CAT activity was set at 1. The results are expressed as the mean plus standard error of the mean.