FIG. 6.

Folding of TBE virus envelope proteins expressed in trans. COS-1 cells transfected with a plasmid coding for prM and E (SV-PEwt) as a polyprotein (expression in cis; PE), with plasmids encoding prM (SV-prM) and E (SV-Ewt) separately (expression in trans; P+E), or with a plasmid coding for E alone (SV-Ewt; E) were pulsed for 2 min and chased for 60 min. Total cellular extracts were immunoprecipitated with either a rabbit anti-prM/E polyclonal antiserum, an anti-E MAb, or a mouse anti-prM MAb as indicated above the lanes, and this was followed by analysis of the proteins by reducing SDS-PAGE. Positions of the proteins are marked at the left, and molecular size standards are indicated on the right in kilodaltons.