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DTT resistance of C-terminally truncated TBE virus E proteins. Cells were transfected with recombinant plasmids as indicated and subjected to a pulse-chase experiment with 2-min pulses and chases of 0 to 60 min. After a chase in normal nonreducing medium, the cells were chased in DTT-containing medium for an additional 4 min. The ratio of DTT-resistant E protein to total E protein was plotted for the Ewt, E472, and E400 forms.
