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Endo Hf sensitivity of TBE virus envelope proteins. Two-minute pulse and 60-min chase samples were immunoprecipitated with a polyclonal antiserum recognizing both prM and E, followed by digestion of the immune complexes with Endo Hf. Subsequently, the proteins were analyzed by reducing SDS-PAGE and visualized by autoradiography. All recombinant constructs plus a sample from virus-infected cells were tested, as indicated at top of the lanes.
