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Induction of apoptotic activities by LGT virus NS3 mutants in Neuro-2a cells. Cells were transfected with plasmids expressing NS3 mutant proteins (A) and/or Casp8-HA (B) and then fixed, stained with X-Gal, and counted to determine the percentage of apoptotic cells at 72 h (A) and 24 h (B) after transfection. Empty vector-transfected cells served as a negative control for apoptosis.
