FIG. 8.

LGT virus NS3 protein contains two caspase-8-binding regions. 293T cells were cotransfected with plasmids expressing Casp8m-HA and F-NS3, Flag-tagged, truncated NS3 mutants, or Flag-tagged Fas ligand. Cell lysates were immunoprecipitated with anti-Flag MAb Sepharose, and bound proteins were separated by SDS-polyacrylamide gel electrophoresis, transferred onto a membrane, and probed with anti-HA.11 MAb to detect coimmunoprecipitating Casp8m-HA (A). Expression of the epitope-tagged proteins in cells was confirmed by immunoblotting aliquots of cell lysates with anti-HA.11 (B) or anti-Flag (C) MAb.