TABLE 1.
Restriction enzyme digestion and sequence analysis of PERV clones
| Polymerase used | Digestion pattern | No. of clones | No. of clones sequenced [ID(s)a] | Most likely type from sequence comparison | Presence of premature stop codon [ID(s)] |
|---|---|---|---|---|---|
| Taq | AK | 1 | 1 (1) | A+B | Yes (1) |
| AM | 10 | 3 (6, 10, 11) | A | Yes (6, 10, 11) | |
| AN | 4 | 2 (8, 82) | A | Yes (8, 82) | |
| AO | 1 | 1 (17) | A | Yes (17) | |
| AP | 4 | 2 (54, 56) | A | Yes (54) | |
| BL | 41 | 6 (3, 4, 9, 12, 14, 20) | B | Yes (3, 12) | |
| BN | 1 | 1 (24) | B | No | |
| CL | 1 | 1 (21) | A+B | No | |
| DL | 1 | 1 (29) | B | Yes (29) | |
| Pfu | AV | 2 | 1 (251) | A | No |
| AM | 15 | 1 (6) | A | No | |
| AN | 17 | 1 (11) | A | No | |
| AX | 1 | 1 (260) | A+B | Yes (260) | |
| AP | 48 | 1 (3) | A | No | |
| AQ | 5 | 1 (56) | A | Yes (56) | |
| AR | 2 | 1 (62) | A | No | |
| AS | 4 | 1 (112) | A | Yes (112) | |
| AT | 1 | 1 (115) | A | Yes (115) | |
| AU | 1 | 1 (232) | A+B | Yes (232) | |
| AW | 1 | 1 (295) | A | Yes (295) | |
| BL | 248 | 1 (1) | B | No | |
| DU | 1 | 1 (345) | A+B | No |
a
ID(s), clone identification number(s).