TABLE 3.
Sequence analyses of 17 clones with premature stop codons
| Clone IDa | Stop codon positionb | Cause(s) of stop codonc |
|---|---|---|
| Taq-1 | 416 | ΔT nt 1134 (frameshift mutation) |
| Taq-3 | 578 | Base substitution C1733T |
| Taq-6 | 467 | ΔA nt 1250 (frameshift mutation) |
| Taq-8 | 416 | ΔT nt 1134 (frameshift mutation) |
| Taq-10 | 330 | ΔC nt 815 (frameshift mutation), ΔT nt 1134 (frameshift mutation) |
| Taq-11 | 428 | ΔT nt 1134, ΔA nt 1250 (frameshift mutation) |
| Taq-12 | 242 | Base substitution A725T |
| Taq-17 | 395 | Base substitution C1184T |
| Taq-29 | 373 | ΔA nt 1042 (frameshift mutation) |
| Taq-54 | 416 | ΔT nt 1134 (frameshift mutation) |
| Taq-82 | 416 | ΔT nt 1134 (frameshift mutation) |
| Pfu-56 | 416 | ΔT nt 1134 (frameshift mutation) |
| Pfu-112 | 416 | ΔT nt 1134 (frameshift mutation) |
| Pfu-115 | 511 | Base substitution C1532T |
| Pfu-232 | 428 | ΔT nt 1134, ΔA nt 1250 (frameshift mutation) |
| Pfu-260 | 416 | ΔT nt 1134 (frameshift mutation) |
| Pfu-295 | 416 | ΔT nt 1134 (frameshift mutation) |
a
ID, identification.\
b
Position of the stop codon in relation to the position of the codon for the first amino acid in the sequence.\
c
Nucleotide (nt) positions are positions of mutations in the nucleotide sequence. The nucleotide numbering starts from the 5′ forward primer sequence (nucleotide 1).