FIG. 2.
TVB32-46 allows ASLV-B infection of receptor-negative cells. TVB32-46 was mixed with the subgroup B virus RCASBP(B)-EGFP, and the virus-peptide complex was then added to cells with (dark shaded circles) or without (light shaded circles) a gentle centrifugation (spinoculation) step. Infection was then quantified by flow cytometric analysis to measure the number of infected (EGFP-positive) cells. Data are means and standard deviations (error bars) from at least four independent experiments, each performed in triplicate. These experiments were performed at a multiplicity of infection of 3 GFP-transducing units (measured by infecting TVB-expressing human 293 cells with the same amount of native virions) as described in Materials and Methods.