FIG. 4.
A chimeric TVA protein containing TVB32-46 binds to both ASLV-A and ASLV-B SU. Chimeric TVA receptors TVA/B and TVA/B*, containing, respectively, either the wild-type TVB32-46 peptide or a triply substituted (L36V Q37L L41P) version, were generated. Human 293 cells that expressed equivalent amounts of either TVA/B or TVA/B* were incubated with either SUA-rIgG (light shaded bars) or SUB-rIgG (dark shaded bars) and a fluorescein isothiocyanate-conjugated secondary antibody and were subjected to flow cytometry. Data are means and standard deviations (error bars) from at least two independent experiments that were performed in triplicate.