FIG. 3.

Infection of nocodazole- and hydroxyurea-arrested cells with ASV. (A) Cell cycle profiles of dividing and nocodazole-arrested DF-1 cells obtained after 24 h of treatment (from a separate experiment). (B) DF-1 cells were synchronized by 24 h of treatment with hydroxyurea (HU) and infected with ASVA-GFP in the presence of nocodazole. Cells were maintained in nocodazole (NZ) for an additional 36 h, at which time micrographs were taken. DF-1 cells were arrested with HU for 24 h, infected for 2 h, and maintained in HU for 48 h. Results with control dividing cultures are also shown. Shown are phase-contrast (left) and fluorescence (right) micrographs of dividing and arrested cells 48 h postinfection.