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. 2002 Jun;76(11):5395–5403. doi: 10.1128/JVI.76.11.5395-5403.2002

TABLE 1.

Oligonucleotides used in this study

Oligonucleotide Position Sequence (5′ to 3′)a
Primers for site-directed mutagenesis
    Egr-1m −169 to −160 TCCCCGCCCAGATATGGAGAAGAAAC
    Etsm −116 to −112 CACTACCGCTCTTTCCAGATGAGCTC
    AP-1/CREm −106 to −99 TTCCTCCAGATCTGTGCATGGGTTTC
    NF-κBm −97 to −88 ATGAGCTCATGGATATCTCCACCAAGG
    AP-1m −65 to −59 TTTCCGCTGGTGTAAGGATTCTTTCCC
    SP-1m −52 to −45 TGATTCTTTCCGGACCCTCCTCTCGCC
    AP-2m −36 to −28 CCTCCTCTCGCCTCATGGACATATAAG
Tandem copies of transcription factor-binding elements
    (AP-1)4 −65 to −59 ctgaacgcgtTGAATGATGAATGATGAATGATGAATGActcgagagtc
    (AP-2)4 −36 to −28 ctgaacgcgtCCCCAGGGACCCCAGGGACCCCAGGGACCCCAGGGActcgcgcgtc
    (NF-κB)4 −97 to −88 ctgaacgcgtGGGTTTCTCCGGGTTTCTCCGGGTTTCTCCGGGTTTCTCCctcgcgcgtc
Probes for EMSA
    AP-1 −65 to −59 TTTCCGCTGGTTGAATGATTCTTTCCC
    AP-2 −36 to −28 CCTCCTCTCGCCCCAGGGACATATAAG
    Egr-1 −169 to −160 TCCCCGCCCCCGCGATGGAGAAGAAAC
    NF-κB −97 to −88 ATGAGCTCATGGGTTTCTCCACCAAGG
    AP-1m −65 to −59 TTTCCGCTGGTGTAAGGATTCTTTCCC
    AP-2m −36 to −28 CCTCCTCTCGCCTCATGGACATATAAG
a

Binding sites are shown in bold type. The linker sequences at the ends of the oligomers are indicated by lowercase letters.