TABLE 1.
Oligonucleotide | Position | Sequence (5′ to 3′)a |
---|---|---|
Primers for site-directed mutagenesis | ||
Egr-1m | −169 to −160 | TCCCCGCCCAGATATGGAGAAGAAAC |
Etsm | −116 to −112 | CACTACCGCTCTTTCCAGATGAGCTC |
AP-1/CREm | −106 to −99 | TTCCTCCAGATCTGTGCATGGGTTTC |
NF-κBm | −97 to −88 | ATGAGCTCATGGATATCTCCACCAAGG |
AP-1m | −65 to −59 | TTTCCGCTGGTGTAAGGATTCTTTCCC |
SP-1m | −52 to −45 | TGATTCTTTCCGGACCCTCCTCTCGCC |
AP-2m | −36 to −28 | CCTCCTCTCGCCTCATGGACATATAAG |
Tandem copies of transcription factor-binding elements | ||
(AP-1)4 | −65 to −59 | ctgaacgcgtTGAATGATGAATGATGAATGATGAATGActcgagagtc |
(AP-2)4 | −36 to −28 | ctgaacgcgtCCCCAGGGACCCCAGGGACCCCAGGGACCCCAGGGActcgcgcgtc |
(NF-κB)4 | −97 to −88 | ctgaacgcgtGGGTTTCTCCGGGTTTCTCCGGGTTTCTCCGGGTTTCTCCctcgcgcgtc |
Probes for EMSA | ||
AP-1 | −65 to −59 | TTTCCGCTGGTTGAATGATTCTTTCCC |
AP-2 | −36 to −28 | CCTCCTCTCGCCCCAGGGACATATAAG |
Egr-1 | −169 to −160 | TCCCCGCCCCCGCGATGGAGAAGAAAC |
NF-κB | −97 to −88 | ATGAGCTCATGGGTTTCTCCACCAAGG |
AP-1m | −65 to −59 | TTTCCGCTGGTGTAAGGATTCTTTCCC |
AP-2m | −36 to −28 | CCTCCTCTCGCCTCATGGACATATAAG |
Binding sites are shown in bold type. The linker sequences at the ends of the oligomers are indicated by lowercase letters.